NSW Nurses and Midwives Association (NSWNMA) members voted in a secret ballot on Tuesday to stop work at Bathurst Base Hospital for 24 hours on Friday, November 29.
The association claims the protest is in opposition to the Western NSW Local Health District's decision to close five hospital beds from December 9.
The strike will take place from 7am (AEDT) on Friday to 7am on Saturday but night-duty staff levels will be maintained to handle emergencies.
The strike is a major step for Bathurst Base Hospital members but they feel they have no other choice, NSWNMA general secretary Brett Holmes says.
"It is an indication of just how serious the ongoing uncertainty over Bathurst hospital and community health services has become," he said in a statement.
Nurses are also worried other hospital and health services will be cut in the Bathurst area, NSWNMA says.
A public meeting will also be held the following Monday, December 2, to canvass the impact of proposed cuts to the hospital.
"The people of Bathurst also deserve to have a say and to be listened to by the Western NSW LHD and state government," Mr Holmes said.
The meeting will be held at the Panthers League Club in Bathurst on Monday from 6pm.